BGN 15: Mapping the barley genome using restriction fragment length polymorphisms (RFLPs) BARLEY GENETICS NEWSLETTTER, VOL. 15, II. RESEARCH NOTES
Shewry, p. 39

II. 17. Mapping the barley genome using restriction fragment length polymorphisms (RFLPs).

P.R. Shewry, Biochemistry Department, Rothamsted Experimental Station, Harpenden, Herts. U.K.

RFLP mapping has a number of advantages over conventional genetic analysis, including a higher degree of polymorphism and the possibility of obtaining an almost infinite number of probes which are evenly distributed over the whole genome. In addition, it is possible to use combinations of probes to following quantitative multigenic characters. The principles and application of RFLP mapping are discussed in detail in several recent review articles (see for example Tanksley, 1983; Rivin et al., 1983).

It is important that workers in this expanding field keep in touch, to avoid duplication and to facilitate the exchange of information and probes. After consultations with several other workers I have agreed to prepare and distribute a list of the specific interests, experimental material and probes (in use and available for distribution) of different research groups. It is hoped that this will develop into an active collaboration.

References:

Tanklsey, S.D. 1983. Molecular Markers in Plant Breeding. Plant Molecular Biology Reporter 1:3.

Rivin, C.J., Zimmer, E.A., Cullis, C.A., Walbot, V., Huynh, T., Davis, R.W. 1983. Evaluation of Genomic Variability at the Nucleic Acid Level. Plant Molecular Biology Reporter 1:9.
 

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