A Database for Triticeae and Avena
As part of a project to produce a map of the barley genome based on
DNA RFLP's, we have obtained cDNA's and genes for characterized proteins
from various laboratories, and are determining their chromosomal
locations using the ditelosomic addition lines of barley (cv. Betzes)
into wheat (cv. Chinese Spring) produced by Islam and Shepherd (1983).
The plants were grown in the glasshouse at Rothamsted and their
karyotype confirmed by root tip analysis. Standard methods for DNA
extraction and RFLP analysis were used which will be reported in detail
Some preliminary results using six clones are summarized in Table 1,
and a typical result shown in Fig. 1.
B=Betzes, CS=Chinese Spring
The locations of the thionin precursor and high pI a-amylase genes on
the short and long arms respectively of chromosome 6 are consistent with
the assignment of the thionin precursor genes to chromosome 6 by Bohlmann
et al. (1988) and with recent RFLP mapping (Kleinhofs et al., 1988;
Wettstein-Knowles, 1989). Similarly Hejgaard et al. (1984) have
previously mapped the gene for BASI to chromosome 2 using isoenzyme
analysis of the wheat/barley addition lines. Loi et al. (1988) showed
that the cDNA encoding (1-3, 1-4) b-glucanase isoenzymne II hybridized to
two restriction fragments in EcoRI digested genomic DNA from cv Betzes,
one of which was located on chromosome 1. The second fragment was not
present in any of the available addition lines of Betzes chromosomes into
Chinese Spring wheat, and was therefore assumed to be located on
chromosome 5. The location of the gene for glutamine synthetase
(chloroplast form) has not been reported previously.
This research was supported by Shell Research Ltd. and Nickerson R.P.B. Ltd.
We are indebted to all the workers referred to above who generously made
their clones available for this study.
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