BGN 19: Further investigation on mutant gene cu4 for curly 4 (spiral) in barley by means of primary trisomic analysis

Further investigation on mutant gene cu4 for curly 4 (spiral) in barley by means of primary trisomic analysis*

S. Wang and T. Tsuchiya
Department of Agronomy
Colorado State University
Ft. Collins, Colorado 80523, U.S.A.
*Supported by USDA-CSU Cooperative Research Project No. 58-82HW-6-8 and CSU Hatch Project.

The mutant gene cu4 for curly 4 (spiral) in KM118 was induced with irradiation by Tsuchiya in 1962. The detailed characteristics and adapted symbol (cu4) were reported by Tsuchiya (1974, 1984a,b). Previous investigations designed to associate the gene with a barley linkage group showed that the gene is not associated with chromosome 1, 3, 4, 5, or 6 (Furst, 1983; Hwang and Tsuchiya, 1988). Additional information with primary trisomic analysis with chromosome 7 is shown in Table 1.

Table 1. F2 segregation of Triplo 7 × spiral (cu4 mutant).
2xF2 segregation ratios
2x + 1Total
+cu4Total +cu4Total +cu4Total x2 (3:1)
292150 15621 4427 711.61

The data from Table 1 showed that there were six mutant homozygotes in 21 trisomics, which indicated the noncritical combination of the gene cu4 with Triplo 7. The gene cu4 is not located on chromosome 7.

Thus, the gene cu4 is most likely present on chromosome 2. Primary trisomic analysis with Triplo 2 is underway.

References:

Furst, E.C. 1983. Primary trisomic analysis of three mutant genes in barley. M.S. Thesis, Colorado State University, CO.

Hwang, J.J., and T. Tsuchiya. 1988. Primary trisomic analysis of the gene cu4 for curly 4 (spiral) mutant in KM118. BGN 18:18-20.

Tsuchiya, T. 1974. Root character of curly mutants in barley. BGN 4:88-89.

Tsuchiya, T. 1984b. Curly 4, cu4. BGN 14:97.

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