BGN 19: Primary trisomic and telotrisomic analyses of yst3 in barley.

Primary trisomic and telotrisomic analyses of yst3 in barley.

S. Wang and T. Tsuchiya
Department of Agronomy
Colorado State University
Ft. Collins, Colorado 80523, U.S.A.


The gene for yellow streak 3 (yst3) is a spontaneous mutant gene found in the variety Lion (Singh and Tsuchiya, 1973) and was described in detail by Tsuchiya (1984). Primary and telosomic trisomic analyses have been conducted on seven different chromosomes since 1976. The results are listed in Table 1.

Table 1.Cross combinations between trisomic types and yellow streak 3 (yst3) mutant types and F2 segregation.

Primary trisomic analysis (Tsuchiya and Alanko, 1986) suggested that the gene yst3 may be associated with chromosome 2. However, telotrisomic analysis for triplo 2L and 2S indicated that the gene yst3 was not associated with chromosome 2 (Tsuchiya and Hang, 1979). Since deficiency was found in the telocentric chromosome 2S in some Triplo 2S materials by Giemsa N-banding (Singh and Tsuchiya, 1982), the telotrisomic analysis for Triplo 2S was conducted again with Giemsa N-banding technique applied. No deficiency was found in the telosome 2S in all Triplo 2S tested. The ratio of F2 segregation confirmed the conclusion by Tsuchiya and Hang (1979) that yst3 was not associated with chromosome 2.

The ratios of F2 segregations so far obtained suggest that the gene yst3 cannot be associated with any chromosome except 7. Recent study concluded that the gene was not associated with the short arm of chromosome 7. Primary trisomic analysis for Triplo 7 is now underway.

References:

Singh, R.J., and T. Tsuchiya. 1973.BGN 3:54-55.

Singh, R.J., and T. Tsuchiya. 1982. Identification and designation of telocentric chromosomes in barley by means of Giemsa N-banding technique. Theor. Appl. Genet. 64:13-24:

Tsuchiya, T. 1984. BGN 14:99.

Tsuchiya, T., and J. Alanko. 1976. BGN 6:84-85.

Tsuchiya, T., and A. Hang. 1979. BGN 9:106-108.

Tsuchiya, T., and A. Shahla. 1982. BGN 12:31-32.


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