BGN 2: The use of infrared photography in characterizing chlorophyll mutants BARLEY GENETICS NEWSLETTER, VOL. 2, III. GENETIC AND CYTOLOGICAL TECHNIQUES
McMullen, pp. 121-122

III.5. The use of infrared photography in characterizing chlorophyll mutants.

Michael McMullen. Department of Agronomy, Colorado State University, Fort Collins, Colorado 80521, U.S.A.

The chlorina character of four Trebi mutant lines, Trebi VI, X, XII and XIII was found to be maternally inherited. It was not possible to distinguish between these lines using an allelism test, but a difference was expressed in the F2 generation between plants resulting from the interaction of a specific homozygous recessive nuclear chlorina gene and the various types of maternal chlorina cytoplasm. Color infrared photography was used to distinguish between these lines. Healthy chlorophyll appears deep red using color infrared photography and abnormal chlorophyll appears a lighter shade of red to white.

Since a difference between normal and abnormal chlorophyll will be shown with infrared photography, it was assumed that a difference between the maternal chlorina Trebi mutants might also be expressed. Using Kodak Ektachrome infrared film and a No. 12 filter, a difference between the maternally inherited chlorina mutants was observed in the resulting slides.

This method may be useful in characterizing other chlorophyll mutants,

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