BGN 2: Coordinator's report on the genetic male sterile barley collection BARLEY GENETICS NEWSLETTER, VOL. 2, IV. REPORTS FROM COORDINATORS
Hockett, pp. 139-144

IV.7. Coordinator's report on the genetic male sterile barley collection.

E. A. Hockett. Plant Science Research Division, ARS, U.S.D.A. and Plant and Soil Science Department, Montana State University, Bozeman, Montana 59715, U.S.A.

Three new numbered loci have been assigned to the genetic male sterile series as shown in Table 1. These results from crossing a homozygous male sterile plant with a heterozygous male sterile plant of the known or previously numbered loci, show that ms22e, ms23b, and ms24v are not allelic with ms1 through 21 or allelic with each other.

Table 2 gives the results of testing for allelism where the mutant lines were found to be allelic with previously designated lines. The ms,, s line is allelic with ms5 and ms,, ak and ms,, an are allelic with ms24 and with each other.

The temporary symbols assigned to new genetic male sterile lines are shown in Table 3. These 34 letter symbols have not been given previously in the literature and supplement Hockett et al. (1) and Hockett and Eslick (2). The symbols ms1 aw, ms2 ax, ms1O ay and ms 11 az have been assigned so that they can be differentiated from the original ms1, ms2, etc. (1,2). Male sterile stock numbers are those assigned to the collection maintained at Bozeman and are a continuation of the list published previously (1). The male sterile stock numbers 102, 103, 104 and 105 assigned to ms,,ak, ms,,al, ms,,am and ms,,an in (2) should be 302, 303, 304 and 305 since numbers 102-105 duplicate the male sterile stock numbers of winter barley lines listed in (1).

All the new lines segregated as expected if a single recessive gene were responsible for the male sterility except ms,,as, ms,,at, ms,, av, and ms,, bt. In all cases there was an excess of plants in the male sterile class when counts were evaluated. According to Sharma (3), ms,,as, ms,,at,, and ms,, av all behaved as single recessive genes in Canada. Sib crosses of ms,, av ms,, av x Ms,, av ms,, av gave a ratio of 9:8 fertile to male sterile plants at Bozeman which fits the 1:1 ratio expected for a single gene with P  80%. Since the sample size for ms,, bt is quite small, further data is needed to resolve the question of a single gene being responsible.

Selfing under glassine bags for these new male sterile lines is also shown in Table 3. No data are presented for ms1 aw, ms2 ax, ms1O ay and ms1l az since it has been reported previously (2). In some cases, the suspected selfed seed has not been grown out for a check on chance outcrossing. Therefore, some of the low percentages reported might be modified with further investigation.

New information on location of the genes on chromosomes is shown in Table 4. The ms6 gene was previously reported to be on chromosome 1 or 7 (2) but it is now known to be on chromosome 6 (Eslick, unpublished data). The ms,, i gene, through a typographical error, was reported to be "not near centromere of any chromosome" in (2) but it is allelic to ms1 (2) so is on chromosome 5. The mutant line intended for Table 4 in (2) was ms,, j and is so listed here. The tentative location of ms,, h is known from unpublished data of Hockett and the ms,, bk location from unpublished data of Ramage and Eslick.

It is requested that all persons with genetic male sterile stocks send them to Bozeman with the information requested in (1).

Table 1. Ratios of fertile to male sterile F1 plants from crosses of genes determined to be nonallelic

Table 2. Ratios of fertile to male sterile F1 plants from crosses of genes determined to be allelic.

Table 3. Ratios of fertile to sterile plants in F2 and selfing behavior of sterile plants.

Table 3, (continued) (p. 143)

Table 4. The location of male sterile genes.

References:

Hockett, E. A., R. F. Eslick, D. A. Reid, and G. A. Wiebe. 1968. Genetic male sterility in barley II.  Available spring and winter stocks. Crop Sci. 8:754-755,

Hockett, E. A., and R. F. Eslick, 1971. Genetic male sterile genes useful in hybrid barley production. Barley Genetics II:308-315. Wash. State Univ. Press, Pullman, Washington.

Sharma, R. K. 1970. Studies of sterility mutants in spring barley (Hordeum vulgare L.), Ph.D. Diss., Univ. of Guelph, 81 p.

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