A Database for Triticeae and Avena
IV.7. Coordinator's report on the genetic male sterile barley collection.
E. A. Hockett. Plant Science Research Division, ARS, U.S.D.A. and Plant
and Soil Science Department, Montana State University, Bozeman, Montana
Three new numbered loci have been assigned to the genetic male sterile
series as shown in Table 1. These results from crossing a homozygous male
sterile plant with a heterozygous male sterile plant of the known or previously
numbered loci, show that ms22e, ms23b, and ms24v are
not allelic with ms1 through 21 or allelic with each other.
Table 2 gives the results of testing for allelism where the mutant lines
were found to be allelic with previously designated lines. The ms,,
s line is allelic with ms5 and ms,, ak and ms,, an
are allelic with ms24 and with each other.
The temporary symbols assigned to new genetic male sterile lines are
shown in Table 3. These 34 letter symbols have not been given previously
in the literature and supplement Hockett et al. (1) and Hockett and Eslick
(2). The symbols ms1 aw, ms2 ax, ms1O ay and ms
11 az have been assigned so that they can be differentiated from the
original ms1, ms2, etc. (1,2). Male sterile stock numbers
are those assigned to the collection maintained at Bozeman and are a continuation
of the list published previously (1). The male sterile stock numbers 102,
103, 104 and 105 assigned to ms,,ak, ms,,al, ms,,am
and ms,,an in (2) should be 302, 303, 304 and 305 since numbers
102-105 duplicate the male sterile stock numbers of winter barley lines
listed in (1).
All the new lines segregated as expected if a single recessive gene
were responsible for the male sterility except ms,,as, ms,,at,
av, and ms,, bt. In all cases there was an excess of plants
in the male sterile class when counts were evaluated. According to Sharma
(3), ms,,as, ms,,at,, and ms,, av all behaved as single
recessive genes in Canada. Sib crosses of ms,, av ms,, av x Ms,,
av ms,, av gave a ratio of 9:8 fertile to male sterile plants at Bozeman
which fits the 1:1 ratio expected for a single gene with P 80%. Since
the sample size for ms,, bt is quite small, further data is needed
to resolve the question of a single gene being responsible.
Selfing under glassine bags for these new male sterile lines is also
shown in Table 3. No data are presented for ms1 aw, ms2 ax,
ay and ms1l az since it has been reported previously (2). In
some cases, the suspected selfed seed has not been grown out for a check
on chance outcrossing. Therefore, some of the low percentages reported
might be modified with further investigation.
New information on location of the genes on chromosomes is shown in
Table 4. The ms6 gene was previously reported to be on chromosome
1 or 7 (2) but it is now known to be on chromosome 6 (Eslick, unpublished
data). The ms,, i gene, through a typographical error, was reported
to be "not near centromere of any chromosome" in (2) but it is allelic
to ms1 (2) so is on chromosome 5. The mutant line intended for Table
4 in (2) was ms,, j and is so listed here. The tentative location
of ms,, h is known from unpublished data of Hockett and the ms,,
bk location from unpublished data of Ramage and Eslick.
It is requested that all persons with genetic male sterile stocks send
them to Bozeman with the information requested in (1).
Table 1. Ratios of fertile to male sterile F1 plants
from crosses of genes determined to be nonallelic
Table 2. Ratios of fertile to male sterile F1 plants
from crosses of genes determined to be allelic.
Table 3. Ratios of fertile to sterile plants in
F2 and selfing behavior of sterile plants.
Table 3, (continued) (p. 143)
Table 4. The location of male sterile genes.
Hockett, E. A., R. F. Eslick, D. A. Reid, and G. A. Wiebe. 1968. Genetic
male sterility in barley II. Available spring and winter stocks.
Crop Sci. 8:754-755,
Hockett, E. A., and R. F. Eslick, 1971. Genetic male sterile genes useful
in hybrid barley production. Barley Genetics II:308-315. Wash. State Univ.
Press, Pullman, Washington.
Sharma, R. K. 1970. Studies of sterility mutants in spring barley (Hordeum
vulgare L.), Ph.D. Diss., Univ. of Guelph, 81 p.
BGN 2 toc
BGN Main Index