A Database for Triticeae and Avena
IV.9. Function and activity of Genetic and Aneuploid Stock Center*.
T. Tsuchiya. Department of Agronomy, Colorado State University, Fort
Collins, Colorado 80521, U.S.A.
* The work reported in IV.9. (p.146 ) - IV 11. (p. 155) has been supported
in part by NSF Research Grant GB4482X and GB 30493, and Colorado State
University Experiment Station Project (Hatch 8).
The Genetic Stock Center in the Agronomy Department, Colorado State
University, Fort Collins, Colorado, U.S.A., had been operated for a long
time by the late Dr. D. W. Robertson. The Center's operation has been supported
by a research grant from the National Science Foundation (NSF) of the United
States of America since 1961 with the contract title "Assembly, Maintenance,
Distribution, and Complementary Research Studies of Genetic and Chromosome
Tester Stocks in Barley". The Stock Center is now operated by T. Tsuchiya
with continuous support from National Science Foundation since 1969.
Since T. Tsuchiya, coordinator of research in trisomics and aneuploids,
joined Colorado State University in 1968, trisomics and other aneploid
stocks are included to the operation of the Center.
In Tables 1-4 is a listing of the number of genetic and trisomic stocks
handled in the Center from 1969 to 1971.
The genetic stocks are periodically grown for increase and for restoration
of viability. In 1969, 422 stocks were grown in the field. In 1970 and
1971, 302 and 401 stocks were grown in the greenhouse. Also, newly received
stocks have been grown in the greenhouse in 1970 and 1971. The number of
stocks grown are shown in Table 5.
Table 1. Genetic stocks sent out.
Table 2. Genetic Stocks Received.
Table 3. Trisomic and aneuploid stocks sent out.
Table 4. Trisomic stocks received.
Table 5. Number of genetic and aneuploid stocks
grown in 1969-1971.
The total number of genetic stocks kept in the Stock Center is about
2000. In the past three years we have been working toward establishing
a system for the maintenance and distribution of genetic and aneuploid
stocks efficiently and effectively. About 1000 stocks have been received
in the stock center in the past three years. The exact number of stocks
will be reported in the next issue of BGN.
It was found that the Stock Center does not have many stocks for designated
genes listed in the Summaries (1941, 1947, 1955, 1966), and the two symposium
reports (1964, 1971) prepared by D. W. Robertson and co-authors and Reviews
by L. Smith (1951) and R. A. Nilan (1964). Effort will be made to collect
as many of these missing stocks as possible in the near future. Cooperation
of barley research workers who have found new mutants and received gene
symbols for them, yet have not sent seeds to the Stock Center, is appreciated.
For old stocks used by some deceased research workers, we will ask research
workers in the same country as those deceased researchers if certain stocks
are still available. Special cooperation from these people will be highly
The other areas of the activity of the Stock Center are (1) allelism
testing among many similar type mutants in our stocks, (2) studies of aneuploids
and (3) the establishment of multiple genetic marker stocks for each arm
of each chromosome.
(1) A large number of mutants have been collected during the past 50
years in the Stock Center. Very little allelism testing had been done until
recently. Some of the results of allelism tests are presented (Tsuchiya,
1971. BGN 1:62 and 1:65; Tsuchiya, 1972, BGN 2:80-87; McMullen and Haus,
1971 BGN1:68-69; MuMullen, 1972. BGN, 2:76). Additional allelism testing
is now underway with various types of mutations. In this way, the number
of stocks to be kept in the Stock Center can be reduced.
All the stocks which have been maintained in the Stock Center will be
stored in the National Seed Storage Laboratory, U. S. Department of Agriculture,
Fort Collins, Colorado, U.S.A. Only one genetic stock for each locus will
be maintained in the Stock Center for distribution and genetic-linkage
The complete list of barley genetic stocks in both the Stock Center
in the Agronomy Department of Colorado State University and the National
Seed Storage Laboratory will be published together with available information
for the stock in the Barley Genetics Newsletter in the near future.
(2 ) It has been well demonstrated that genetic and linkage studies
by means of trisomic analysis is a most effective and efficient method.
The Barley Genetic Stock Center here in Fort Collins is also the center
of stock maintenance and research with primary trisomics, teletrisomics
and other aneuploids in barley. Primary trisomics and telotrisomics have
been and are being used for associating new mutant genes with a chromosome
(primary trisomic analysis) or with a chromosome arm (telotrisomic analysis)
(Tsuchiya, 1971, BGN 1:61-62; 1972, BGN 2:93. In addition to this a large
number of plants from 329 F1 combinations and 172 F2 populations were grown
in 1969 to 1971. An extensive telotrisomic analysis is now underway in
the Stock Center. A total of 47 marker genes on five chromosomes (1-5)
have been or are being tested by means of telotrisomic analysis. At least
10 or more marker genes, most of which are lethal chlorophyll mutations,
located or associated with the five non-satellited chromosomes will be
studied by means of telotrisomic analysis. These results will give rather
precisely the centromere position for each of those five chromosomes in
the near future.
(3) Once the centromere position is located in the linkage maps in relation
to the marker genes, then it will become easy to establish multiple marker
stocks for each arm of, at least, the five nonsatellited chromosomes.
BGN 2 toc
BGN Main Index