BGN 2: Isolation of high protein strains of barley BARLEY GENETICS NEWSLETTER, VOL. 2, II. RESEARCH NOTES
Bansal, pp. 21-22

II.1. Isolation of high protein strains of barley.

H. C. Bansal. Division of Genetics, Indian Agricultural Research Institute, New Delhi-12, India.

The discovery of the Hiproly gene (Munck et al., Proc. II Int. Barley Genetics Symp. 1969) and the isolation of the "Notch mutant" (Bansal, Curr. Sci. 39, 1970) has indicated the feasibility for increasing the protein content as well as changing the amino acid profile in barley. The hulled and shrivelled grains in the notch mutant were found to have a high dye binding capacity (DBC) value (0.310) and high protein content (18.48%) as compared to the values 0.20 and 13.93, respectively, in the mother variety (NP 113). The notch character was found to be simply inherited as a recessive factor and the high protein trait was stable in different environments.

Determinations of DBC values on single plants in the two notch mutants and three strains showed that the notch-l mutant was similar to the Hiproly barley as far as DBC values and 1000 kernel weights were concerned, while the crude protein content was slightly higher in Hiproly (Table 1). Minn 90-5 strain obtained from Dr. T. Tsuchiya in U.S.A., has a marker for the smooth awned character on chromosome 7. This strain was also found to show high DBC value (0.29), being similar to that of the notch-2 mutant. Thus, Minn 90-5 has also been identified as a high protein (19.14%) strain of barley. The shrivelling in the grains of mutants and Minn 90-5 was similar to Hiproly (Fig. 1) except that the grains were hulled, unlike the naked grains of Hiproly.

Table 1. Protein content and DBC values of the parent (NP 113), its mutants and some other strains of barley, grown side by side.

BGN 2 toc
BGN Main Index