BGN 2: Linkage studies on a gene for high lysine content in Hiproly barley BARLEY GENETICS NEWSLETTER, VOL. 2, II. RESEARCH NOTES
Karlsson, pp. 34-36

II.9. Linkage studies on a gene for high lysine content in Hiproly barley.

K. E. Karlsson. Swedish Seed Association, S-268 00 Svalöf, Sweden.

High lysine content in Hiproly (CI 3947) is controlled by one recessive major gene (lys) (Munck et al., 1970). To locate this gene, Hiproly was crossed with different marker stocks having genes on all the chromosomes except chromosome 3. The F2 plants were classified morphologically and chemically for lysine. The lysine content was determined according to the DBC method (Mossberg, 1969).

Linkage was established in two crosses with s in the long arm of chromosome 7 (Table 1). The crosses, set up in coupling, gave somewhat askew segregation ratios in F2, demanding for a careful interpretation of map distances. The two crosses, analyzed two different years, were made in different genetic backgrounds (Bonus and Kristina). The technique for lysine analysis was slightly different in the two years.

Persson (1969) reported s and lax-a to be linked (14%). Lys and lax-a are inherited independently (Table 1) indicating lys to be located about 8 units distally to s (Table 2).

Further investigations are under way for a more definite localization of lys, as well as existing modifiers.

Table 1. Crosses between Hiproly and genetic markers.

Table 2. Segregations in crosses between Hiproly (sslyslys) and Bonus and Kristina respectively (SSLysLys).

References:

Mossberg, R. 1969. New approaches to breeding for improved plant protein. Proc. Panel Rostanga 1968. IAEA Vienna 1969, 151-160.

Munck L., Karlsson K.-E., Hagberg, A. and Eggum, B. 1970. Science 168:985-987.

Persson, G. 1969. Hereditas 63:1-28.

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