BGN 2: Effect of temperature on chlorophyll content of induced viable chlorina mutants of Himalaya barley BARLEY GENETICS NEWSLETTER, VOL. 2, II. RESEARCH NOTES
Kleinhofs and R. L. Warner, pp. 41-42

II.11. Effect of temperature on chlorophyll content of induced viable chlorina mutants of Himalaya barley.

A. Kleinhofs and R. L. Warner. Department of Agronomy and Soils, Washington State University, Pullman, Washington 99163, U.S.A.

It has been often observed, that temperature has a strong effect on the phenotype of some barley chlorina mutants. We were interested in identifying the temperature sensitive mutants among our mutant collection. The mutants were grown at 10° and 26° C. The primary leaf was assayed for chlorophyll content 7 days after planting in the 26° C regime and 17 days after planting in the 10° C regime. At this time the primary leaf was fully expanded. The data is presented in Table 1.

Table 1. Chlorophyll content of some viable barley chlorina mutants grown at 10° C and 26° C.

It can be observed that there are some mutants that are extremely cold sensitive. Some of these appear to be normal to the eye when grown at 26° C. Some of the mutants are not affected by temperature and some are heat sensitive. At both extremes, the mutants appear to be conditionally lethal.

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