BGN 2: Genetic studies of induced viable chlorina mutants of Himalaya barley BARLEY GENETICS NEWSLETTER, VOL. 2, II. RESEARCH NOTES
Millham and Kleinhofs, pp. 53-54

II.19. Genetic studies of induced viable chlorina mutants of Himalaya barley.

Betty Jo Millham and A. Kleinhofs. Department of Agronomy and Soils, Washington State University, Pullman, Washington 99163, U.S.A.

A number of viable chlorophyll - deficient barley mutants are being tested for their mode of inheritance and allelism among themselves and with the collection of located chlorina mutant stocks, f, fc, f2, f4, f5, f6, f7, f8, f9. The mutants were induced in the variety Himalaya by chemical or radiation treatments and are identified by an abbreviation of the treatment and an arbitrary number. Abbreviations used as dES - diethyl sulfonate, EMS - ethyl methane sulfonate, iPMS isopropyl methane sulfornate, g - gamma irradiation, S - spontaneous, and Unk - unknown.

The preliminary data indicates that all of the mutants tested so far segregate in a typical Mendelian manner.

Allelism test, although incomplete, show only one suspected case of allelism; i.e. in the cross dES 18/dES 19. In this case three chlorina F1 seedlings were obtained. Maternal inheritance can be ruled out since dES 18 has been used as the female parent in other crosses without any indication of maternal inheritance. Further testing is required to differentiate between allelism and selfed seed.

No indication of maternal inheritance has been observed when mutants dES 1, dES 9, dES 18, dES 19, dES 20, EMS 11, Unk 3, and g 1 were used as the female parent. The search for maternal inheritance has been hampered by the extreme female sensitivity of the mutants in the Himalaya background.

Several of the mutants have been crossed to the located chlorina mutant stocks and F1 plants examined. Again no allelism has been found. All of the F1 plants were normal green. This data is summarized in Table 1. The known chlorina mutants were used as the female parent and the induced mutants as the male parent. The numbers indicate the numbers of F1 plants observed.

Table 1. Results of intercrossing chlorina mutants with designated chlorina stocks. Numbers indicate number of F1 green plants for each cross.

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