BGN 2: Frost hardiness in spring barley and negative heterosis for this character in a cross of spring x winter barley BARLEY GENETICS NEWSLETTER, VOL. 2, II. RESEARCH NOTES
Schwarzbach, p. 74

II.29. Frost hardiness in spring barley and negative heterosis for this character in a cross of spring x winter barley.

E. Schwarzbach, Technische Universitat Munchen, Institut fur Pflanzenbau und Pflanzenzuchtung, 8050 Freising, Weihenstephan, Deutschland.

In experiments with seedlings of the spring barley 'Wisa' and the winter barley 'Tria' at the coleoptile stage the plants were completely killed when frozen at -6°C without previous hardening. Afcer 2 days hardening at 1°C in the dark the survival after freezing at -8°C reached nearly 100%, when killing was defined as the absence of regrowth during a 2 day recovery period of seedlings, cut at a height of 15 mm above the kernel basis. The results indicate, that genes for frost hardiness can be present also in a spring barley.

In a cross of the winterhardy variety 'Dura' with the spring barley marker lines of 'Bonus' the frost hardiness of the F2 population was investigated. Seedlings were germinated in the dark to the stage, at which the first leaf penetrates the coleoptile. After 4 days hardening in the dark at 1°C the temperature was lowered at a rate of 1°C/h to -12°C and maintained at this level for 8 hours The frost damage was determined by measuring the electrical resistance of seedling torsos, produced by cutting coleoptiles and rootlets 15 mm above and below the kernel. The frost damage was highly significantly increased in the F2 population compared to both parents, the mean electrical resistance being about 12, 15 and 22 kiloohms for the F2 population, the 'Bonus' line and the winter barley 'Durap'. It seems possibly, that hybrid vigor in early generations interferes with the hardening process.

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