GrainGenes
A Database for Triticeae and Avena
Ramage and Clark, p. 58
II.28 Translocation T2-3ag.
R. T. Ramage and D. R. Clark. Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721, U.S.A. "R"
A shrunken endosperm mutant, labeled B-71-AJ-72, was sent to us by R. F. Eslick. In allele tests with other shrunken endosperm mutants, all Fl's involving the line B-71-AJ-72 were partially sterile. At metaphase I of meiosis, the F1's had a ring of four chromosomes plus 5 pairs indicating that B-71-AJ-72 was homozygous for a translocation.
The line B-71-AJ-72 was crossed with the translocations T1-6a, T1-7a, T2-4a, T3-5a and T4-5a. F1's with T1-6a, T1-7a and T4-5a had two rings of 4 plus 3 pairs at metaphase I of meiosis. F1's with T2-4a and T3-5a had a ring of 6 plus 4 pairs at metaphase I.
The metaphase I configurations exhibited by the Fl's indicate: (a) that chromosomes 1, 4, 5, 6 and 7 are not involved in the translocation and (b) that the translocation in the line B-72-AJ-72 is between chromosomes 2 and 3. The translocation has been designated T2-3ag. Examination of chromosomes in root-tips of T2-3ag revealed no discernable differences in chromosome morphology.
The line B-71-AJ-72, which is homozygous for both the shrunken endosperm mutant seg6f and the translocation T2-3ag, has been crossed with normal 'Betzes' to separate to shrunken endosperm mutant from the translocation.