from Bob Graybosch, 1/96
 
 
                      About PNW Regional Quality Data
 
      PNW Regional Quality Data contains the results of wheat quality analyses
 conducted by the USDA-ARS Western Wheat Quality Laboratory, Washington State
 University, Pullman, WA.  Samples analysed were entrants in the USDA-ARS
 Regional Performance Nurseries; the present file contains data from 1981-94.
 Four data files are presented.  File 1 contains information on nursery, year,
 pedigrees, entry identification (ID number) and market class of wheat.  ID
 number is used to identify samples in the remaining files.  File 2 contains
 information on wheat milling performance, protein contents, and mixograph
 characteristics.  File 3 presents cookie, sponge cake, noodle, and breadbaking
 characteristics.  File 4 contains farinograph and rapid viscoanalyzer data.
 Not all tests are conducted on all samples, dependent upon the market class of
 wheats.  A list of abbreviations and description of materials and methods is
 given below.  Special thanks are due to Doug Engle and Craig Morris, USDA-ARS,
 Pullman, WA for providing data.
 
                               DESCRIPTION OF ABBREVIATIONS
 
 AWRC      Alkaline water retention capacity
 BABS      Bake water absorption (percentage by weight, corrected to 14% 
              moisture basis)
 BCRGR     Bread crumb grain rating code
 BFYELD    Break flour yield (percentage flour from break rolls by weight of
              total products)
 %BFYELD   Break flour yield (percentage flour from break rolls by weight of 
              total products, short flow method)
 %BRAN     Bran recovered (percentage bran from break rolls by weight of the 
              total products, short flow method)
 BREEDER # Plot or bag number within a nursery
 CAVOL     Japanese sponge cake volume (cc)
 CLASS     Hardness, color, and season planted (market class, subclass)
 CNCOL     Cooked noodle color score
 CODI      Cookie diameter (cm)
 COLOR     Alkaline  flour color rating 
 DSI       Cibacron blue dye test for alpha-amylase
 FABS      Farinograph water absorption (percentage by weight, corrected to 14%
               moisture basis)
 FASH      Flour ash (percentage by weight, corrected to 14% moisture basis)
 FMIST     Flour moisture (percentage by weight)
 FN        Falling number test for sprout damage
 FPEAK     Farinograph mixing peak time (minutes)
 FPROT     Flour protein (percentage by weight, corrected to 14% moisture basis)
 FSTAB     Farinograph stability time (minutes)
 FYELD     Flour yield (percentage flour by weight of total products)
 LECOFPROT Flour protein content
 LECOWPROT Wheat protein content (see Appendix B)
 LOCATION  Location sample was grown
 LVOL      Bread loaf volume (cc)
 MABS      Mixograph absorption (percentage by weight, corrected to 14% 
              moisture basis)
 %MIDDS    Unground middling stock (percentage middlings from break rolls by 
              weight of the total products, short flow method)
 MSCOR     Milling score
 MTI       Farinograph mixing tolerance index (see Appendix B)
 MTIME     Optimum mixing time for bread dough (minutes)
 MTYPE     Mixograph type (see Appendices B and C)
 NIRFMIST  Flour moisture (see Appendix B)
 NIRFPROT  Flour Protein (see Appendix B)
 NIRWPROT  Wheat Protein (see Appendix B)
 NOSCOR    Noodle score (scale 1-100, see Appendix B)
 NURSERY   Nursery  number (located in the upper left corner of the tables)
 NYELD     Noodle yield (weighted score assigned to WTIN)
 OVENFMIST Flour moisture 
 %PFYELD   Potential flour yield equal to break flour and unground middling 
              stock (percentage by weight of the total products, short flow
              method)
 PROQ      Protein quality
 REG NURS  Regional Nursury
           - SW         Spring Wheat
           - WWW        White Winter Wheat
           - HSW        Hard Spring Wheat
           - HRWW       Hard Red Winter Wheat
           - HRSW       Hard Red Spring Wheat
           - SWSW       Soft White Spring Wheat
 RNCOL     Raw noodle color score
 RVA       Rapid Visco-Analyzer (peak starch paste viscosity, centipoise X 10)
 SAMPLE #  Sample number
 SCSOR     Sponge cake score (scale 1-100)
 SKHRD     Single kernel hardness
 SKHRDSD   Single kernel hardness standard deviation
 SKMST     Single kernel moisture
 SKMSTSD   Single kernel moisture standard deviation
 SKSIZE    Single kernel size
 SKSIZESD  Single kernel size standard deviation
 SKWT      Single kernel weight
 SKWTSD    Single kernel weight standard deviation
 TEXTNO    Cooked noodle texture score 
 TGS       Cookie top grain score (scale 0-10)
 TWT       Test weight (lbs/bu, after cleaning but before scouring)
 UWHRD     Udy cyclone mill wheat grain hardness (NIR value, dimensionless)
 VARIETY   Variety name, state number or unique identifying number
 VISC      Predicted McMichael viscosity (observed modified Brookfield 
              viscosity x 7.5)
 WMIST     Wheat moisture (percentage by weight)
 WPROT     Wheat protein (percentage by weight, corrected to 12% moisture basis)
 WTIN      Noodle weight increase (percentage water uptake after cooking)
 YEAR      Year sample harvested
 
 
 
 
                           METHODS
 
 Sample Preparation: The wheat samples are logged into the WAS computer system
 and assigned a Nursery code number (NURSERY), which includes SAMPLE #,
 LOCATION, BREEDER #, VARIETY, and YEAR.  All wheat samples are frozen at -27 C
 for 2 d to kill insects, cleaned on a Kice aspirator, measured for test weight
 and then scoured and sub-sampled for proximate analysis.  Test Weight (TWT):
 Determined by AACC Method 55-10 (1).
 
 Udy Wheat Hardness (UWHRD): The standard FGIS NIR wheat grain hardness
 value.Uses the
 
 Udy Cyclone mill with a 0.5-mm screen as the standard grinder
 (AACCMethod39-70A).
 
 Single Kernel Analysis: 300 kernels are individually analyzed using a
 SingleKernelWheat Characterization System (SKWCS) commercial prototype (Model
 P3) made by Perten Instruments.  The mean and standard deviation from the 300
 kernel sample are reported for four parameters.  They are hardness, size,
 moisture, and weight.  Themachine works by weighing (SKWT) each kernel prior
 to it being dropped between a rotor and crescent which crushes it (ow!).  The
 crescent has a load cell attached which will sense the kernels size (SKSIZE),
 the force (SKHRD) needed to crush the kernel, and the moisture(SKMST) of each
 kernel.  The rotor is ribbed to sweep the crushed kernel past the crescent
 preparing the machine for the next kernel.
 
 Buhler Milling: All of the samples of advanced and regional nurseries
 aremilled on aBuhler MLU-202 pneumatic laboratory mill (Fig. 1).  The samples
 are tempered to a predetermined moisture content ranging from 14.0% to 16.0%,
 depending on the hardness and known flour-bolting properties.  The harder
 wheats require more water.  Thus, the grain is conditioned so that the most
 rapid and complete separation of endosperm can bemade.  The temper solution
 contains a wetting agent (0.1% Aerosol OT) to hasten moisture penetration.
 The wheat is allowed to temper for 16-24 hours before milling topermit uniform
 distribution of the moisture.  An additional 0.5% water is added 15-20 minutes
 before milling.  The first and second break and first and second reduction
 streams predict long patent flour yield.  All six flour streams are then
 combined tomake a straight-grade white flour by sifting on a 120 stainless
 steel wire screen and thoroughly blending.
 
 Flour Yield (FYELD): The percentage by weight of the total products recovered
 as straight-grade white flour.
 
 Break Flour Yield (BFYELD): The percentage by weight of the total products
 recovered as flour off the break rolls.
 
 Milling Time: The time required in minutes to mill a 2-kg sample with
 theBuhler experimental mill and obtain a normal separation of bran, shorts,
 and flour. Time is dependent on adjustments made by an experienced miller
 after visually observing themilling properties.
 
 Milling Score (MSCOR):  Calculated as follows:    
 
 MSCOR = 100-[(80-flour yield)+50(flour ash-.30)+0.48(milling time-12.5)+
   0.5(65-per cent long patent)+0.5(16-first tempering moisture)]
 
 Modified Quadrumat Milling: The preliminary nurseries (250-500 g) are
 experimentally milled on a Quadrumat System as modified by Jeffers and
 Rubenthaler (11).  The procedure is described in the 27th Annual Report, Oct.
 1976 (Pgs. 1-14).  Conversion of the data to give a predicted Buhler flour
 yield and milling score are done with the following linear equations:
 
      Predicted Buhler Milling Score 
       Soft Wheat  (y = -21.60185 + 1.27367 x)    
       Hard Wheat (y = - 3.43818 + 1.0448 x)     
 
      where, y = Predicted Buhler flour yield and x = Original Quadrumat flour
 yield Modified Quadrumat Milling Short Flow: Wheat samples (approximately
 40-100 g size)are atmospherically conditioned (tempered) by placing the wheat
 (in envelopes) into a constant temperature and relative humidity chamber.
 Moisture content is raised to the desired level (13.0% - soft wheat and 14.5%
 - hard wheat).  The conditionedwheat is ground only through the Quadrumat
 break rolls.  The standard sifting schedule used in the Modified Quadrumat SR.
 milling procedure is followed.  Break flour produced from hardwheat is usually
 25 - 30% and from soft wheat about 45 - 50%.  This amount of flour produced is
 generally sufficient for certain physio-chemical predictivetests that require
 littleflour ie., mixogram, Rapid Visco Analyzer viscosity, Brookfield
 viscosity or AWRC.
 
 Bran (%BRAN):  The amount of bran recovered.
 
 Break Flour Yield (%BFYELD): The percentage by weight of the total products
 recovered as flour off the break rolls.
 
 Midds (%MIDDS):  Unground middling stock.
 
 Potential Flour Yield (%PFYELD): The potential flour equal to break flour and
 unground middling stock.
 
 Wheat Moisture (WMIST) & Flour Moisture (FMIST): The reference test uses 2 g
 of ground wheat heated in an aluminum dish in a forced-draft oven for 40 min
 at 130 C, allowed to cool and weighed.
 
 Flour moisture (OVENFMIST) is determined in the same manner except that it is
 heated only 20 min (1, modified Method 44-16).  The NIRspectrometer (Technicon
 450) (NIRFMIST) is routinely used and is calibrated to the above method.
 
 Flour Ash (FASH): The percentage ash from a 4-g sample of flour ignited and
 heated for 15 hours at 550 C in a muffle furnace (1, Method 08-01).
 
 Wheat Protein (WPROT) and Flour Protein (FPROT): Protein concentration is
 determined by NIR (NIRWPROT and NIRFPROT). Minor corrections are made based on
 the results from about 10% of the samples analyzed using the combustion method
 (Leco,model FP-428) (LECOFPROT and LECOWPROT). (1, Method 46-30).
 
 Alkaline Water Retention Capacity (AWRC): The per cent increase in weight of
 7.5 g of flour due to absorption of water from 35 mL of 0.1 N NaHCO3 solution
 after centrifugation (18) (1, Method 56-10 with proportionate decrease to 5 g
 flour).
 
 Viscosity (VISC): Dial reading x 7.5 of a RVT Brookfield
 Synchro-LectricViscometer fitted with a modified No. 2 spindle at 50 R.P.M.
 using a suspension of 20 g of flour in 100 ml of water and 7 ml of 1 N lactic
 acid (15).
 
 Rapid Visco Analyzer (RVA): A hot-pasting viscosity measured by the Rapid
 ViscoAnalyzer.  The viscosity reported is the peak viscosity (centipoise X
 10)and is obtained at 93 C after 2 min of pregelatinization at 65 C.  It
 emulates the Brabender Amylograph.  Foreign users have suggested that an
 Amylograph viscosity value (no tanalyzed here) of 500 Brabender Units (BU or
 AU) is a minimum value, and 1000 or greater is a desirable value for noodle
 quality.  RVA and Amylograph peak viscosity values have a linear correlation
 of about 0.99.  Therefore, minimum RVA values would be near 125 with values
 above 210 desirable.
 
 Falling Number (FN): Determined by AACC Method 56-81B.Cibacron Blue (DSI):
 Units: DU/g (dextrinizing units/g).  Directly measures alpha-amylase activity
 through the liberation of Cibacron blue dye covalently linked to a beta-limit
 dextran substrate.
 
 Mixogram: Used to characterize wheats as to market class and estimate mixing
 and baking properties of flours.  The 10-g instrument is used and the testing
 procedure and interpretation of K.F. Finney (9) is followed.  To reduce the
 time and expense involved in reproducing the mixograms, a reference chart was
 developed to characterize each curve as to type ranging from very weak to
 exceptionally long and strong types.
 
 Farinograph: The Farinograph is equipped with a 50-g bowl and the Constant
 Flour Weight procedure (14% moisture basis) is employed (1, Method 54-21A).
 
 Farinograph Absorption (FABS): The amount of water required to center
 thehighest portion of the Farinograph curve on the 500 Brabender unit (BU)
 line.
 
 Farinograph Mixing Peak Time (FPEAK): The time interval, in minutes, from the
 firs taddition of water until the curve reaches its maximum height.
 
 Farinograph Stability Time (FSTAB): The number of minutes the top of thecurve
 remains above the 500 unit line when the highest portion (peak) is centered on
 the500 unit line.
 
 Farinograph Mixing Tolerance Index (MTI): The difference in Brabender
 Units(BU) from the top of the curve at peak mixing time to the top of the
 curve fiveminutes after the peak nixing time.
 
 Cookie Baking (yum!): The micro method employs 40 g flour at 25% absorption,
 60%sugar,30% non-emulsified shortening, 3% dry skim milk, 1% NH4HCO3, 1% NaCL,
 and 1%NaHCO3 and 0.24% emulsifier (distilled mono- and di-glycerides).
 
 Cookie Diameter (CODI):  The average diameter, of one cookie (cm).
 
 Top grain score (TGS): A visual evaluation score describing the top grain of
 the sugar snap cookie.  Range of 0 to 10, with 10 being the best.
 
 Bread Baking: An optimum absorption, optimum mixing, 90 minute fermentation
 straight dough method using 100 g flour, 1.8% dry yeast, 1.5% salt, 6% sugar,
 0.3%malt extract,4% dry milk solids, 75 ppm ascorbic acid, and 3% partially
 hydrogenatedshortening with mono-and di-glycerides (Crisco) (5,6,7,10).
 
 Bake Water Absorption (BABS): The amount of water required to make a dough of
 proper consistency for bread baking when mixed to optimum conditions as judged
 by an experienced baker using the baking method described above.
 
 Mixing Time (MTIME): Time in minutes required to mix the flour and the other
 breaddough constituents to the optimum condition as judged by an experienced
 baker.
 
 Loaf Volume (LVOL): Volume of bread loaf, measured by canola seed displacement
 (cc).
 
 Bread Crumb Grain (BCRGR): Subjective judgement of crumb grain quality as
 judged by an experienced baker.
 
 Protein Quality (PROQ): An assessment of the protein quality for breadbaking.
 The loaf volume of experimental lines is compared to a historical regression
 that predicts loaf volume based on protein content.  If loaf volume of the
 experimental line is +/- 25 cc of thehistorical, then PROQ = 0.  If loaf
 volume of the experimental differs from the predicted loaf volume by more than
 25 cc, then a "+1" or "-1" is generated.  This new parameter replaces the
 previous X-Y scatter plot of protein vs. loaf volume with the historical and
 nursery regression lines.  Currently the regression is of the form y=ax+b,
 where loaf volume (y)= flour protein (x) times 65 (a,slope) plus 140
 (b,intercept), or LVOL =FPROT(65) + 140.
 
 Japanese Sponge Cake Baking: Formula contains 100 g flour, 100 g sugar, 100g
 fresh egg, 40 g water.
 
 Cake Volume (CAVOL): Volume displacement of sponge cake, measured with canola
 seeds (cc).
 
 Sponge Cake Score (SCSOR): Additive score of texture, volume, external factors
 and crumb grain (open-ended).  The standard flour produces a cake with a score
 equal to 80.
 
 Udon Noodle Making: Formula contains 300 g flour, 6.0 g NaCl, 96 g water (14%
 constant flour moisture basis) with dough sheet and noodles prepared with an
 Otake Laboratory Noodle Machine.  Often, optimum water absorption is used as
 opposed to constant absorption.
 
 Noodle Yield (NYELD):  Relative score assigned to WTIN (see below).
 
 Noodle Score (NOSCOR): Additive score incorporating raw and cooked noodle
 color, yield and texture.
 
 Noodle Weight Increase (WTIN): Percentage weight increase of cooked noodle
 relative to raw noodles.
 
 Raw Noodle Color Score (RNCOL): The score subjectively assigned to raw noodles
 which were assessed visually.  The standard control raw noodle is assigned a
 score of 16.  Scores assigned to the experimental raw noodle can range from
 1-20.
 
 Cooked Noodle Color Score (CNCOL): The score subjectively assigned to cooked
 noodles which were assessed visually.  The standard control raw noodle is
 assigned a score of 16.  Scores assigned to the experimental cooked noodle can
 range from 1-20.
 
 Cooked Noodle Texture Score (TEXTNO): The score assigned by a sensory panel
 which considers factors such as chewiness, firmness and/or softness,
 springiness,elasticity, and other bite characteristics.  The standard control
 cooked noodle is assigned a score of 32.  Scores assigned to the experimental
 cooked noodles can range from 1 to 40.
 
 Alkaline Flour Color (COLOR): An alkaline Pekar slick is prepared by pressing
 flour to a smooth surface, immersing in a 3.0% alkaline solution consisting of
 50 % K2CO3 and 50%Na2CO3 for 2 minutes.  The sample rests at a room
 temperature of 77 F for 4 hours before visual observation and scoring.  Flours
 were scored by the following: S =satisfactory, Q= questionable, and U =
 unsatisfactory.  Desirable flour color ranges from bright white through creamy
 yellow to bright yellow.  Any dullness, brown, or greenish casts
 areunacceptable.  Alkaline flour color is scored only on SWW, HWW, SWS and HWS
 flours which have a minimum RVA viscosity of 150 and a minimum NIR wheat
 hardness (UWHRD) value of 40.0.