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GrainGenes Marker Report: Xbcd721

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Locus
Xbcd721
Type
RFLP
Map
Avena-Sorrells-C40.2
[ Show Nearby Loci ]
Map Data
Diploid Oat, Cornell2
Probe
BCD721
Homology
AC022492_19e-value: 2e-23(AC022492) F1L3.35 [Arabidopsis thaliana]
AC022492_19e-value: 1e-47(AC022492) F1L3.35 [Arabidopsis thaliana]
Possible Orthologs
BCD721



Probe for Locus
BCD721
Locus
Xbcd721
BCD721
Synonym
SynonymHAR35_2493
HAR35_50722
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Type
cDNA
Sequence
BE438680
BE438770
BCD721_WHE1F0061
BCD721_WHE1F0062
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
PCR size
0.8
Clone Vector
pBluSK
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Data Source
Sorrells, Mark E.96.07