Query (optional)   in Class  

GrainGenes Probe Report: BCD452

[ Printable Version ]  [ Submit comment/correction ]

Probe
BCD452  [ Marker Report ]
Locus
Xbcd452-3A
BCD452
BCD452B
Xbcd452
bcd452
Synonym
SynonymHAR35_21343
Reference
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Lambda ZAP II library
Bar.LambZAPII [c]
Type
cDNA
Sequence
BE438629
BE438679
BE438768
BCD452_WHE1E0071
BCD452_WHE1E0072
Polymorphism
BCD452 XbaI
Linkage Group
3L
Insert Enzyme
EcoRI
Source Species
Hordeum vulgare
Source Germplasm
Willis
Source Tissue
etiolated leaf
Insert Size
0.9
PCR size
0.9
Clone Vector
pBluSK
pBluSK-
Excision Enzyme
EcoRI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 78 sec, 50 C 78 sec, 72 C 120 sec, 25 times; 94 C for 78 sec, 50 C 78 sec, 72 C 5 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
BCD452 SO autoradiogram
Data Source
Sorrells, Mark E.96.07
ATMI92.09