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GrainGenes Probe Report: CDO1473

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Probe
CDO1473  [ Marker Report ]
Locus
Xcdo1473c
[ Show all 13 ]
Reference
ReferenceWight CP et al. (2004) Discovery, localization, and sequence characterization of molecular markers for the crown rust resistance genes Pc38, Pc39, and Pc48 in cultivated oat (Avena sativa L.) Molecular Breeding 14:349-361.
ReferenceHeun M et al. (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare) Genome 34:437-447.
General Remarks
Uni-ZAP XR library
Type
cDNA
Sequence
BE439324
BE439356
CDO1473_WHE05H07.s1
CN180773
Polymorphism
CDO1473 EcoRV
Source Species
Avena sativa
Source Germplasm
Brooks CIav9260
Source Tissue
etiolated leaf
PCR size
1.5
Clone Vector
pBluSK
Excision Enzyme
EcoRI
XhoI
Vector PCR primers
M13forward
M13reverse
Vector Amplification
94 C for 60 sec, 50 C 40 sec, 72 C 60 sec, 34 times; 94 C for 60 sec, 50 C 40 sec, 72 C 10 min, 1 time
Antibiotic
Ampicillin
Clone Location
Sorrells, Mark E.
GrainGenes Probe Repository
Clone Authority
Sorrells, Mark E.
Image
CDO1473 KO autoradiogram
Data Source
Sorrells, Mark E.96.07