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GrainGenes Probe Report: WMC238

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC238  [ Marker Report ]
Locus
Xwmc238
Xwmc238-4B
wmc238
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 5f2
Donor: Sharp, Camden
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 181 bases located on 4B, 227 bases located on 4B
Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 87-270 match part of 5B12, and part of 5g10. (CA)22 96 to 139. Reverse primer is outside of homology with 5b12.
Type
PCR
SSR
PCR primers
Forward TcTTccTgcTTAcccAAAcAcA at 70 length 22 bases, Tm 64
Reverse TAcTgggggATcgTggATgAcA at 272 length 22 bases, Tm 69
SSR size
224 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC238
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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