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GrainGenes Probe Report: WMC332

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Probe
WMC332  [ Marker Report ]
Locus
Xwmc332-2B
[ Show all 6 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 17b12
Donor: Khairallah, Mexico
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 172 bases located on 2B
Trimed to insert at both ends. (CT)12 136 to 159.
Type
PCR
SSR
PCR primers
Forward cATTTAcAAAgcgcATgAAgcc at 51 length 22 bases, Tm 65
Reverse gAAAAcTTTgggAAcAAgAgcA at 198 length 22 bases, Tm 62
SSR size
169 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (several loci).
Sequence
WMC332
Source Species
Triticum aestivum
Data Source
Somers, Daryl J.2004.06
Isaac, Peter G.2004.06

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