The 676 translocation lines induced by X-irradiation and selected at Gatersleben were added to the World Collection (Tables 1 to 3).

The 415 lines with single translocations are compiled in Table 1. The lines were selected and confirmed as described earlier (Künzel and Scholz, 1978; Künzel et al., 1984). For the assignment breakpoints to chromosome arms or more specified chromosome regions, see the annotations for Table 1. Giemsa banding of meiotic metaphase I chromosomes in F₁ hybrids of "intercrosses" between lines with the same two chromosomes involved in translocations proved to be especially useful in assigning breakpoints to individual arms (Künzel, 1987).

The designation of chromosomes and chromosome arms follows the proposal of Singh and Tsuchiya (1982), i.e. with respect to the nearly metacentric chromosome 1, the interstitially Giemsa-banded arm is designated as the short (S) arm despite being physically somewhat longer than the arm without an interstitial band (Linde-Laursen 1985).

At present, the World Collection harbors about 1000 lines with single translocations. Summarizing the results of Hagberg (1986), Linde-Laursen (1984, 1987, 1988), Kakeda and Yamagata (1991), and Künzel (cf. Table 1), 390 breakpoints are localized in defined segments on the basis of Giemsa-banded somatic metaphoase chromosomes: 24 in chromosome 1 (short arm 13, centromere region 0, long arm 11); 34 in chromosome 2 (14, 2, 18); 47 in chromosome 3 (17, 3, 27); 68 in chromosome 4 (36, 2, 31); 67 in chromosome 5 (33, 4, 30); 75 in chromosome 6 (satellite 12, Nucleolus Organizing Region 4, short arm segment between NOR and centromere 23, centromere region 3, long arm 33) and 75 in chromosome 7(12, 6, 21, 4, 32), respectively.

Some 261 multiple translocation stocks isolated at Gatersleben are listed in Table 2 and 3. Their karyotypes are restructured by two up to nine translocations. The stocks were produced either by repeated cycles of X-irradiation and selection of additionally induced translocations (Table 2) or, for some special purposes, by crossing of lines with pre-existing translocations (Table 3). All of the multiple translocation stocks are structurally homozygous, i.e., they show 7 bivalents in meiosis after selfing. When crossed with a line of standard karyotype, they will give an F₁ showing the chromosome configurations as listed in the third column of Tables 2 and 3.

Table 1:

Single translocations from Gatersleben
added to the World Collection

Table 2:

Multiple translocation stocks from Gatersleben
added to the World Collections
Developed by repeated cycles of X-irradiation

Table 3:

Multiple translocation stocks from Gatersleben
added to the World Collection Stocks of hybrid origin

Small seed samples of the lines compiled in Tables 1 to 3 can be obtained from G. Künzel.

References:

Georgiev, K., K. Gecheff, H. Nicoloff, G. Künzel, and R. Rieger. 1985. Giemsa banding as a means of identification of reconstructed chromosome in barley. Biol. Zentralblatt 104:29-34.

Hagberg, A. 1986. Induced structural rearrangements. p. 17-36. In: Genetic Manipulation in Plant Breeding, Walter de Gruyter, Berlin.

Kakeda, K., and H. Yamagata. 1991. Localization of breakpoints in barley reciprocal translocations involving chromosome 4 by banding techniques. Barley Genetics VI, Vol. T 2X()-282.

Künzel, G. 1987. Giemsa banding of barley chromosomes in meiosis improves identification of chromosome arms involved in translocations. BGN 17:83-86.

Künzel, G., and F. Marthe. 1991. Segmental interchanges in barley: state of analysis. Barley Genetics VI, Vol. I:283-286.

Künzel, G., and H. Nicoloff. 1979. Further results on karyotype reconstruction in barley. Biol. 7.entralblatt 98:587-592.

Künzel, G. and F. Scholz. 1978. Development and utilization of multiple translocations in barley. p. 112-118. In Experimental Mutagenesis in Plants, Proc. Int. Symp. Varna 1976, Publ. House Bulgar. Acad. Sci., Sofia.

Künzel, G., M. Gramatikova, and S. Hamann. 1984. Isolation of radiation-induced translocations in spring and winter barley. Biol. Zentralblatt 103:649-653.

Linde-Laursen, I. 1984. Breakpoints localized to chromosome arm or region in 26 translocation lines of barley using Giemsa C-banding. BGN 14:12-13.

Linde-Laursen, I. 1985. Cytology and cytogenetics of Hordeum vulgare and some allied species using chromosome banding techniques. Riso Report no. 529, 56 pp.

Linde-Laursen, I. 1987. Localization of breakpoints in 28 translocations involving barley chromosomes 3 and 5 using Giemsa C-banding. BGN 17:101-102.

Linde-Laursen, I. 1988. Giemsa C-banding of barley chromosomes V. Localization of breakpoints in 70 reciprocal translocations. Hereditas 108:65-76.

Nicoloff, H., and G. Künzel. 1976. Reconstructed barley karyotypes as a tool in the analysis of intrachromosomal distribution of chromatid aberrations. Barley Genetics III:236-241.

Nicoloff, H., M. Anastassova-Kristeva, G. Künzel, and R. Rieger. 1977. The behavior of nucleolus organizers in structurally changed karyotypes of barley. Chromosoma 62:103- 109.

Schubert, I., and G. Künzel. 1990. Position dependent NOR activity in barley. Chromosoma 99:352-359

Singh, R. J., and T. Tsuchiya. 1982. Identification and designation of telocentric chromosomes in barley by means of Giemsa N-banding techniques. Theor. Appl. Genet. 64:13-24

Acknowledgement:

The work with single translocations was partly supported by the Federal Ministry for Research and Technology (Grant No. 0319960A).

For adding the collection of balanced tertiary trisomics developed at Gatersleben to the World Collection, an appropriate description will be prepared for the next volume of BGN.